August/14 October 2009

From 2009.igem.org

1. min prep

sample      conc. 
 81              15 ng/uL
 81              24
 81              40
 73              39
 84              40
 73              35
 79              37
 79              30
 82              38
 67              28
 83              35
 73              52
 83              23
 79              28    
 75              62
 86              56
 88              29
 89              15
 89              54
 89              15

2.dation

K204097

Vector		Insert
67	5	76	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	33	dH₂O	36
total	50uL	total	50uL

K204098

Vector		Insert
67	5	77	2
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	33	dH₂O	36
total	50uL	total	50uL

K204099

Vector		Insert
c9	3	69	3
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	35	dH₂O	35
total	50uL	total	50uL

K204100

Vector		Insert
c1	2	69	3
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	36
total	50uL	total	50uL

K204101

Vector		Insert
76	2	2-16L	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	30
total	50uL	total	50uL

K204102

Vector		Insert
77	2	2-16L	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	30
total	50uL	total	50uL

↓
37 degree , 2hr
gel cut & purification
ligation

3.min prep

sample    conc.
 72            64  ng/uL
 72            41
 72            35
  91          153
  91          201
  91          126

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