August/24 August 2009

From 2009.igem.org

1. LB+Amp plate



2.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)
     7-1          27.8   ng/uL
     8-1          23.2
      
     9             21.1
     10-A         29.1
     10-B         25.1
     11-A         15.7
     11-B         34.1
     


3.transformed check
Checked the cell cultures transformed and plated out yesterday (8/23) for colony formation and made an approximate count of the number of colonies.

(sample No.) (no. of colonies)          
    1-4E            ~5
    1-8E             0
    2-16L            1
    2-16N           ~15
    2-18H            2
    
  ligation sample 
      12             0
      13             0
      14             0
      15             0
      
       5             0

 to iquid medium(5mL + Amp 5uL)


4.ligation check

sample5
XbaI1
PstI1
No.22
dH2O11
total20uL


37 , 2hr

gel electrophoresis
Osaka090824.jpg

result

7 (RBS+cinR)
7-1     OK

8 (RBS+LuxI)
8-1     OK

9 (RBS+LasR+terminater)
9       fialure?

10 (RBS+EspE+terminater)
10     ???

11(p(tetR)+RBS+GFP+terminater)
11-B    OK

No. 9 10 → ligation rapid check

back to NOTES