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EPF-Lausanne/19 October 2009
From 2009.igem.org
Contents |
Wet Lab
A new gel was made to assess if the double transformants really weren't (the last gel was a bit fuzzy). 1% 100ml (done yesterday, stayed in TBE buffer overnight), so 190 V for 30 min. The result isn't better than yesterday.
Put RO1.1 and RO2.4 transformants in DH5alpha, RO1.1 or RO2.4 with mutated BB ( ILE427PHE) double transformants in DH5alpha to grow in 5ml LB
Colony PCR of : RO1.1 + BB1 mutated clone #3,6 RO2.4+BB1 mutated clone #4,6
People in the lab
Basile, Gabriela, Tú
