EPF-Lausanne/27 July 2009

From 2009.igem.org

Contents

27 July 2009





Wet Lab

LOVTAP gene have been amplified again and runned on a gel to be sure to have the correct insert.

BBa_B0015 (terminator) was digested, runned on a gel (which displayed the correct band) and extracted with the gel extraction kit, this step didn't worked well.

LOVTAP gene have been than digested, purified and runned on a gel.

We already had a digested BBa_B0015 (terminator).

Finally we ligated overnight at 4°C the digested LOVTAP in both terminators, the one digested-extracted today and the one already digested-extracted.


People in the lab

Gab, Christian, Mélanie, Nicolas, Nath