IBB Pune/3 August 2009
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experimenting with plasmid isolation protocols
- Add glucose to solution 1 in Birnboin and Dolly.
- ReSuspend plasmid in DI water instead of TE
- Increase Lysozyme from 25mg/ml to 100mg/ml
- Decrease incubation time with solution 2
- Make fresh Phenol-Chloroform.
- Go back to Chen and Liu, adn Kado and Olsen protocols.
phew, by the end of it we are left wiping sweat from our brows....Retrieved from "http://2009.igem.org/IBB_Pune/3_August_2009"