IBB Pune/3 August 2009

From 2009.igem.org





experimenting with plasmid isolation protocols


  • Add glucose to solution 1 in Birnboin and Dolly.
  • ReSuspend plasmid in DI water instead of TE
  • Increase Lysozyme from 25mg/ml to 100mg/ml
  • Decrease incubation time with solution 2
  • Make fresh Phenol-Chloroform.
  • Go back to Chen and Liu, adn Kado and Olsen protocols.


phew, by the end of it we are left wiping sweat from our brows....