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IBB Pune/8 October 2009
From 2009.igem.org
I] Assembly.
Digestions:- ES Constitutive promoter Promoter RBS YcdB
XP LacO mRFP GFP RBS
BKBN A2 from fGFP
ligations Constitutive promoter + LacO Promoter RBS + mRFP Constitutive promoter + RBS YcdB + GFP Promoter RBS + GFP
II] PCR of YcdB with M13f and M13r
III] LuxI (AHL) assay with FeCl3 and hydroxylamine. FAILED :{
IV]
Transformation of ligated samples
