Lab July 27 2009

From 2009.igem.org

Checked plates - growth on all 4 plates


new DH5alpha plates look contaminated. Staph? Not sure.


old competent cells look OK. Should we just go back to our old cell line? It seems the issue was a plasmid dilution issue rather than a competent cells issue.


re-plating with the following plasmid dilutions:


currently have 23ng/ul, to get to 10ng/ul mix 4.35ul plasmid and 5.65 ul dH2O


1 ng = 1ul of previous mix in 9ul of dH2O


.01 ng = 1ul of previous mix in 9ul of dH2O


after mixing each plasmid concentration, follow the Transformation Protocol.



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