September/12 September 2009

From 2009.igem.org

1.colony check

   (ligation sample No.)  (no. of colonies)
           24                      0
           29                      0
           36                      0
           37                      0
           38                      0

2.ligation and ligation check

UP   :  24A 24B 29 33A 33B 34A 34B 35B 40A 40B marker 24 1-23L
DOWN :  14A 14B 22A 22B 41A 41B 42A 42B marker 30 30 2-18F 36 1-14N

ligation check : 24 , 33 , 34 , 40 , 22 , 41 , 42 OK!

3.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)
     27              147.2
     28               96.1
     39              108.8

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