September/13 September 2009

From 2009.igem.org

1.Min prep
Result of Nanodrop concentration check

  (sample No.)      (concentration)
    3-1M               27.6 ng/uL
    No. 30            162.0 ng/uL
    No. 36             67.6 ng/uL


2.digestion and ligation

digestion with restriction enzyme


K204047

VectorInsert
1-6I10No. 246
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O6dH2O10
total20uLtotal20uL


K204048

VectorInsert
1-1D5No. 335
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O11dH2O11
total20uLtotal20uL


3.ligation check

Osaka090913.jpg
UP : ligation sample No.47 No.48
DOWN : for ligation check 14A 14B 27 28 29 30 35B 36 39


No. 47 , 48 gel purification & litaion 16 degree O/N

ligation check : failure ???


4.transfromation

for DNA modification : No.41 , No.42 , 1-1K , 1-16I
transformation  : No.45 , No.46 , 1-4K , 1-16C
37 degree O/N


5.digestion and ligation

digestion with restriction enzyme


K204017

VectorInsert
1-6I4No.98
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O12dH2O18
total20uLtotal20uL


K204037

VectorInsert
1-14N101-8K2
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O6dH2O14
total20uLtotal20uL


K204038

VectorInsert
1-14N101-8I2
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O6dH2O14
total20uLtotal20uL

37 degree O/N


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