September/15 September 2009

From 2009.igem.org

1.colony check

sample No.   no. of colony
   14             +++
   17              0
   47              +
   48              1
  2-24I           ++
   45              0
   46              0
  1-14N           +++

2.ligation

K204045

Vector		Insert
1-8I	6	No.31	12
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	10	dH₂O	4
total	20uL	total	20uL

K204046

Vector		Insert
No.32	12	1-8K	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	4	dH₂O	10
total	20uL	total	20uL

34-I,V , 45-I,V , 46-I,V

 UP   : 20-I,V , 27-I,V , 28-I,V , ladder
DOWN  : 29-I,V , 30-I,V , 35-I,V , ladder

gel cut
purification
ligation 16degree O/N

3.transfromation

1-1D , No.19 , 2-18F
No.17 , 45 , 46 , 37 , 38 , 1-2M , 1-8I

for DNA modification

No.41 , 42 , 18 , 1-1K , 1-16I

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