September/16 September 2009

From 2009.igem.org

1. colony check

 sample No.   no.of colony
     17            0
     45            0
     46            0
     48            0
    
    1-1D          +++  
    2-18F         +++
     19           +++
 
    1-8I          +++
    1-2M          +++
     17            3
     38            0
     45            0
     46            0
     
     20           >50
     27           >10
     28           >50
     29            0
     30           >10
     35            3
  
for DNA modification
    1-1K          +++
     41           +++
     42           +++
    1-16I         +++
     18           +++

2.digation for ligation

K204049

Vector		Insert
1-6O	6	1-8O	12
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	8	dH₂O	4
total	20uL	total	20uL

49-I,V , 1-14A , 2-24I , 14A , 48 , 47B
14 , 47 : OK

3.Mini prep

result of nano drop

   sample no.      conc.
     1-1K m+       71.8 ng/uL
     1-4K          21.6 ng/uL
     1-16C         27.8 ng/uL
     1-16I m+      46.1 ng/uL
      18 m+        49.3 ng/uL
      41 m+        58.4 ng/uL
      42 m+        83.2 ng/uL

4.transfromation

No. 45 , 46 , 49

5. ligation quick check

 UP   : 17-A,B,C,D , 20A,B , 27-A,B , 1-6I , ladder , 35-A,B,C , vector
DOWN  : 29-A,B , 2-18H , 30-A,B , 2-18F , laldder , 48-A,B , vector , 31-A,B

6.Mini prep
result of nano drop

 sample No.         conc.
   1-8I           11.2 ng/uL
   1-2M           12.7 ng/uL
   1-1D           10.2 ng/uL
   2-18F          13.6 ng/uL

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