September/23 September 2009

From 2009.igem.org

1. colony check

 sample no    no. of colony
    22             100<
    25             100<
    40             100<
    47             100<
   X1-A            50<
   X2-B             2
   X1-B             3
   X3-B             2
   X2-A             50<
   1-12M            10<
   X3-A             10<
    37              0
    39              0


2.min prep
result of nano drop

 sample no      conc.
   1-2M          42 ng/uL
   1-12M         61
   X1-B          47
   X2-A          98
    38          234
   X3-B          88
    36A         247
    36B          23
   X2-B          96
   X1-A          61
   X3-A          64


3.digation

X4-A

VectorInsert
32161-12M4
SpeI1XbaI1
PstI1PstI1
No.2 Buffer2No.22
dH2O0dH2O12
total20uLtotal20uL


K204052

VectorInsert
1-6I10334
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O6dH2O10
total20uLtotal20uL


K204029

VectorInsert
2-18F82-16L8
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O8dH2O8
total20uLtotal20uL


K204037

VectorInsert
3-1M101-8K3
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O6dH2O13
total20uLtotal20uL


K204039

VectorInsert
3-1M82-8E3
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O8dH2O13
total20uLtotal20uL


K204046

VectorInsert
32131-8K2
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O3dH2O14
total20uLtotal20uL


37°C, 2hr


4.trandfromation

sample no
 28 , 30 , X2-A , X1-B

5.prepared Lb/Amp plate


6.digation

X5

VectorInsert
1-6O81-12M8
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O8dH2O8
total20uLtotal20uL


X6

VectorInsert
1-12A121-12M8
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O8dH2O8
total20uLtotal20uL


X7

VectorInsert
1-12C81-12M8
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O8dH2O8
total20uLtotal20uL


X8

VectorInsert
1-16G121-12M8
SpeI1XbaI1
PstI1PstI1
No.22No.22
dH2O4dH2O8
total20uLtotal20uL



37°C, 2hr
gel electrophoresis
gel cut
purification

ligation



7.digation
K204053

VectorInsert
3-1M8336
SpeI1XbaI1
PstI1PstI1
No.22No.210
dH2O8dH2O8
total20uLtotal20uL

7.transfromation

sample no.
 X4-A , X4-B , 52
 29 , 37 , 39 , 46
 3-1M , 11


back to NOTES