September/8 September 2009

From 2009.igem.org

1.colony check

( sample No.)  (no. of colonies)
      1-1D                100<
      10-D                200~
        -E                200~
       
         9                0
        24                0
        27                1
        28                1
        29                0
        30                0

2.Min prep
Result of Nanodrop concentration check

(sample No.) (concentration)          
    18-A           8.5
      -B           16.5
     
    23-A           19.0
      -B           28.6
     
     22            17.4
     22            15.6
       
     25-A          13.3
       -B          20.2

3.digestion and ligation , and ligation check

K204033

Vector		Insert
1-23L	5	No.19	10
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	11	dH₂O	6
total	20uL	total	20uL

K204034

Vector		Insert
2-6E	10	1-12M	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	14
total	20uL	total	20uL

K204035

Vector		Insert
1-2M	2	1-14L	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	14	dH₂O	10
total	20uL	total	20uL

K204036

Vector		Insert
1-14N	10	1-12M	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	14
total	20uL	total	20uL

↓
37degree , 3hr
↓
Agarose Gel Electro-phoresis

UP   : No.5 , 18A , 18B , 22 , 23A , 23B , 25A , 25B , 26 , ladder 
DOWN : No.33 , 34 , ladder

↓
16 degree O/N

4.digestion and ligation

K204037

Vector		Insert
1-14N	10	1-8K	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	14
total	20uL	total	20uL

K204038

Vector		Insert
1-14N	10	1-8I	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	14
total	20uL	total	20uL

K204039

Vector		Insert
1-14N	10	2-8E	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	14
total	20uL	total	20uL

K204040

Vector		Insert
1-6I	8	23A,B	10
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2 Buffer	2	No.2	2
dH₂O	6	dH₂O	6
total	20uL	total	20uL

↓
37degree , 3hr
↓
Agarose Gel Electro-phoresis

UP   : No.35 , 36 , 37 , 38 , ladder
DOWN : No.39 , 40 , 40 , ladder

↓
16 degree O/N

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