Gel Extraction

What you will need:

• Clean scalpel
• Transilluminator
• Eppendorf tube
• Gel extraction kit
• 55degC water bath
• Vortex
• Microfuge

Procedure

• Place the gel on the transilluminator. Put on face shield and gloves.
• Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
• Pry out the gel ‘cube’ and place it in an eppendorf tube.
• Follow instructions in kit manual.