Team:Alberta/Project/Ligation

From 2009.igem.org

University of Alberta - BioBytes










































































































Ligation

What you will need:

  • Vector
  • Insert
  • Milli Q buffer
  • 5X ligase buffer
  • T4 DNA ligase

Procedure

Volumes of vector and insert may vary but a typical 20 ul reaction might be

  • milliQ water 11 ul
  • 5x ligase buffer 4 ul
  • vector (200 ng/ul) 1 ul
  • insert (200 ng/ul) 3 ul
  • ligase 1 ul
    • Incubate at R/T for 3 hrs or overnight at 12-16 C

Retrieved from "http://2009.igem.org/Team:Alberta/Project/Ligation"