Plasmid Miniprep

What you will need:

• Overnight culture
• Qiagen plasmid miniprep kit

Procedure:

• Transfer 1.5 ml of culture to Eppendorf.
• Spin down at 6000 rpm.
• Discard supernatant and follow procedure in kit handbook
• Determine concentration of purified plasmid with NanoVue spectrophotometer in G311.

Notes

• Low speed pellet is easier to resuspend.
• Step 2 in Qiagen, the alkaline lysis with buffer P2, should be brief to avoid damaging DNA.