Team:Brown/Notebook Meetings/6-16-09

From 2009.igem.org




Team Tasks- June 16, 2009


  • Team 1: EV131- Indu, Ahmad, Minoo
  • Team 2: S.aureus- Eli, Flora, Will
  • Team 3: Bistable toggle- Michael, Ashley, Steph
  • Tasks on hand:
  • Team 1:
    • Posted on google groups
    • Transformation done→ need to grow them up
    • Ordered primers
    • Sequence protein?
  • Sit down and write down protocols
    • Put it in pnotat vector; transform
    • Affinity tests- Keq
  • Team 2:
    • S.aureus→ go to biosafety, find strain, lab regulators today: 164 Angell Street
    • TJ Foster→ email reg. signaling tag
    • Talk to labs that have used S.aureus
    • Quorum kill switch
    • Directed evolution of histamine signal transduction prokaryotic gram+ system
    • Encapsulation
  • Team 3:
    • BioBricks are in registry except Pxyl/TetO→ contact TJ Foster (check for sequence)
    • Construction of model circuit
    • Read Collins paper (methods!!!)