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Team:Brown/Notebook Meetings/8-17-09
From 2009.igem.org
iGEM Meeting
8-17-09
SFH 218
9 AM
- Team 1 update:
- Today they are making the Blotto solution with 6-His
- Purchased Qiagen nickel column purification kit
- After protein comes down in a nickel column
- Tests for histamine binding; selective binding?
- Possible ideas for after binding test; Will suggests Barnea lab competitive binding assay involving GPCR => fluorescence
- Adrian suggestion: Look at crystal structure, and binding pocket; see if you can cut the rest of the protein off and make minimized histamine binding domain
- Degradation mechanism?
- Team 2 update:
- “Chugging right along” all this before Thursday
- Signal peptide construct finished in expression vector for S. epi
- Agr promoter construct in S. epi
- Checking Trg-EnvZ ligations tonight
- S. epi is resistance to Tet
- Looger sent us designs to modify Taz to a histidine receptor; then will move to histamine receptor
- Team 3 update:
- Mutagenic PCR….
- Administrative
- Bio 1950 - Fill out proposal form on bio website
- Planning in advance
- Practice presentation in front of people.
- Google calendar? Weekly lab meetings
- Wiki has to be done a week before the week its due
- Cleaner lab book
- Clean wiki
- Mutagenic PCR
