Team:Cambridge/Parts

From 2009.igem.org


Parts Submitted to Registry

Registry Code Type Sequence Description / Notes Length
BBa_K274001 Reporter MelA. The gene (melA) codes for a tyrosinase which produces a dark brown pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. 1844bp
BBa_K274002 Reporter Violacein. Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites. 7346bp
BBa_K274003 Reporter Vio operon ABDE. Produces a dark green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioC gene removed by restriction digest with BamHI and BglII. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site. 6032bp
BBa_K274004 Reporter Vio operon ABCE. Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction digest with BglII and BclI. This sequence contains four genes, vioA, vioB vioC and vioE, each preceded by their own ribosome binding site. 6200bp
BBa_K274100 Composite CrtEBI with rbs. This Composite Biobrick is created by standard assembly of 3 basic Biobricks coding for enzymes CrtE, CrtB and CrtI (each with rbs). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. 3385bp
BBa_K274110 Generator CrtEBI under constitutive promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under constitutive promoter R0011. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). 3448bp
BBa_K274120 Generator CrtEBI under pBad promoter. This Biobrick is created by putting enzyme cassette CrtEBI (with individual rbs) of Part BBa_K274100 under arabinose-induced promoter I0500. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone). 4603bp
BBa_K274200 Composite CrtEBIY with rbs. This Composite Biobrick is created by standard assembly of 4 basic Biobricks coding for enzymes CrtE, CrtB, CrtI and CrtY (each with rbs). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. 4555bp
BBa_K274210 Generator CrtEBIY under constitutive promoter. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under constitutive promoter R0011. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). 4618bp
BBa_K274220 Generator CrtEBIY under pBad promoter. This Biobrick is created by putting enzyme cassette CrtEBIY (with individual rbs) of Part BBa_K274200 under arabinose-induced promoter I0500. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). 5773bp
BBa_K274111 Composite CrtY under Pbad promoter (I0500). This construct is created by putting BBa_K118013 (rbs+CrtY) under the control of Pbad promoter (I0500), by standard assembly. When used in double-transformation together with a constitutive lycopene-producing device (e.g. BBa_K274110), the resulting system can produce colour change (red to orange) upon induction with arabinose. 2380bp
BBa_K274370 Composite PoPS -> P2 ogr - PF promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PF phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 473bp
BBa_K274371 Composite PoPS -> P2 ogr - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
BBa_K274374 Composite PoPS -> P2 ogr - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 475bp
BBa_K274375 Composite PoPS -> P2 ogr - PLL promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the P2 ogr phage activator and the PLL phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
BBa_K274380 Composite PoPS -> PSP3 pag - PF promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PFF phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 473bp
BBa_K274381 Composite PoPS -> PSP3 pag - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
BBa_K274382 Composite PoPS -> PSP3 pag - PP promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the PP phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 474bp
BBa_K274384 Composite PoPS -> PSP3 pag - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PSP3 pag phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 475bp
BBa_K274391 Composite PoPS -> PhiR73 pag - PO promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PO phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp
BBa_K274392 Composite PoPS -> PhiR73 pag - PP promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PP phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp
BBa_K274394 Composite PoPS -> PhiR73 pag - Psid promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the Psid phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 502bp
BBa_K274395 Composite PoPS -> PhiR73 pag - PLL promoter -> PoPS. This Biobrick is a sensitivity tuner composed of a combination of the PhiR73 pag phage activator and the PLL phage promoter. It is a PoPS converter that alters the sensitivity of an upstream promoter, and the PoPS output displays sigmoidal behavior depending on the PoPS input. 501bp




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