Team:Chiba/Notebook/Calendar/21 September 2009
From 2009.igem.org
(20_September_2009 <|>22_September_2009)
Transformation(1)-1
21:00
We Picked Biobrick parts.
BBa_E2060(mCherry)
BBa_T9003(LacZ alpha protein generator)
21:10
We introduced each plasmids into competent cells.
E2060, T9003 --> Cm resistant competent cells
pCIA3-LuxR and plux-GFP, plux-GFP only --> K resistant competent cells
21:30
We added 120 μL of SOC medium each sample and cultured these at 37 degrees Celsius.
22:30
We planted each sample and cultured these at 37 degrees Celsius.
To judge character of LuxR mutants (1)-1
We poured 1 mL of LB-Amp, Cm liquid medium in 96 deep well and added glycerol stocks.
22:20-
We cultured and shook it at 37 degrees Celsius.