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Team:Chiba/Notebook/Calendar/26 September 2009
From 2009.igem.org
(25_September_2009 <|>27_September_2009)
Contents |
E.coli Painting(3)
 RFP, GFP. CFP |
Examine limit of AHL generation(2)-2
Yesterday's operation is here.
- Today's operation
11:35-
We transplanted E.coli to NC filter and put these on each plates.
And then we took pictures every 30 min.
Picture are here.
Pictures
11:35
 Negative Control |
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 3 |
 8 |
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12:05
 Negative Control |
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 8 |
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12:35
 Negative Control |
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 3 |
 8 |
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13:05
 Negative Control |
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 2 |
 3 |
 8 |
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13:35
 Negative Control |
 1 |
 2 |
 3 |
 8 |
 4 |
 5 |
 6 |
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14:05
 Negative Control |
 1 |
 2 |
 3 |
 8 |
 4 |
 5 |
 6 |
 7 |
14:35
 Negative Control |
 1 |
 2 |
 3 |
 8 |
 4 |
 5 |
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15:05
 Negative Control |
 1 |
 2 |
 3 |
 8 |
 4 |
 5 |
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 7 |
Making colors
PCR
- Template
1 : ECFP(BBa_I6057)
2 : mCherry(BBa_E2060)
3 : V-YFP(BBa_K084003)
4 : mOrange(BBa_E2050)
5 : LacZ α(BBa_T9003)
6 : mRFP without Nco1 site
14:45
We drew up mixtures for PCR.
- Element of mixtures
| Template | 1 μL |
| Primer(F) | 2.5 μL |
| Primer(R) | 2.5 μL |
| Buffer(x 10) | 5 μL |
| dNTP | 4 μL |
| DNA pol. | 1 μL |
| dH2O | 34 μL |
| Total | 50 μL |
15:20-
PCR
Gel electrophoresis
15:30
We made gel for electrophoresis.
agar 0.14 g
TAE 20 mL
17:25-
Gel electrophoresis(135 V, 27 min)
