From 2009.igem.org
Spin tubes #1-8 for 3 minutes
Combined tubes #1-4 and tubes #5-8
Added 300x TENS
Mix by inversion
Added 150x sodium acetate
Mix by inversion
Spin for 2.5 minutes
Transferred Sup to clean tube
Added 1mL of EtOH (ethanol)
Spin for 5 minutes
Wasted pellet in 70% EtOH 300x
Spin for 5 minutes
Resuspend 30x TE
Digest
20x DNA
3x Buffer
5x dH2O
2x EcoRI
"
