"
Team:Gaston Day School/6 May 2009
From 2009.igem.org
- Transform pNICE ~ E. coli/dt>
- 50x competent cells on ice (Carolina Biol.)
- 200x cold 50mM CaCl2
- 10x 50ml pNICE (Lindow lab)
- Ice 15' 8:11-->
- Heat shock 42C 90"
- Add 250x LB
- 1 hour 37C
- Plate 100x, 100,x 200x, 200-250x
- Overnight 37C
