| Component
| Description
| Part or Accession #
| Base Pairs (bp)
| Plasmid (backbone)
| Resistance
| Well
| Quality control
|
| GVP
| Gas Vesicle Proteins
| BBa_I750016
| 6064
| BBa_J61035
| Ampicillin/Gentamycin
| plate 3, pos.1
| ?
|
| GVP (2nd batch)
| Gas Vesicle Proteins
| BBa_I750016
| 6064
| BBa_J61035
| Ampicillin/Gentamycin
| plate 3, pos.2
| ?
|
| pHigh in low copy nr vector
| High consitutive promoter
| BBa_J23100
| 35
| pSB3K3
| Kanamycin
| Plate 3, pos. 4
| PCR(?), restriction(+)
|
| pMed in low copy nr vector
| Medium consitutive promoter
| BBa_J23106
| 35
| pSB3K3
| Kanamycin
| Plate 3, pos. 5
| PCR(?), restriction(+)
|
| pLow in low copy nr vector
| Low consitutive promoter
| BBa_J23109
| 35
| pSB3K3
| Kanamycin
| Plate 3, pos. 6
| PCR(?), restriction(+)
|
| pHigh in high copy nr vector
| High consitutive promoter
| BBa_J23100
| 35
| pSB1AC3
| Ampicillin/Chloramphenicol
| Plate 3, pos. 7
| PCR(?), restriction(+)
|
| pMed in high copy nr vector
| Medium consitutive promoter
| BBa_J23106
| 35
| pSB1AC3
| Ampicillin/Chloramphenicol
| Plate 3, pos. 8
| PCR(?), restriction(+)
|
| pLow in high copy nr vector
| High consitutive promoter
| BBa_J23109
| 35
| pSB1AC3
| Ampicillin/Chloramphenicol
| Plate 3, pos. 9
| PCR(?), restriction(+)
|
| Promotor (low expression)
| Constitutive promotor
| BBa_J23109
| 35
| BBa_J61002
| Ampicillin
| plate 3, pos.10
| No
|
| Promotor (medium expression)
| Constitutive promotor
| BBa_J23106
| 35
| BBa_J61002
| Ampicillin
| plate 3, pos.11
| No
|
| Promotor (high expression)
| Constitutive promotor
| BBa_J23100
| 35
| BBa_J61002
| Ampicillin
| plate 3, pos.12
| No
|
| Inducible promoter
| Lactose inducible promoter nr1
| BBa_R0010
| 200
| pSB1A2
| Ampicillin
| Plate 3, pos. 14
| PCR(?), restriction(+)
|
| Inducible promoter
| Lactose inducible promoter nr2
| BBa_R0010
| 200
| pSB1A2
| Ampicillin
| Plate 3, pos. 15
| PCR(?), restriction(+)
|
| RBS
| Ribosomal Binding Site
| BBa_B0034
| 12
| pSB1A2
| Ampicillin
| plate 3, pos.19
| No
|
| Terminator
| Double terminator consisting of BBa_B0012 and BBa_B0011
| BBa_B0014
| ~90
| pSB1AK3
| Ampicillin/Kanamycin
| plate 3, pos.20
| No
|
| Const. Promoter + GVP
| Low constitutive promoter with GVP cluster
| BBa_J23109 + BBa_I750016
| ~6100
| pSB1AC3
| Ampicillin/Chloramphenicol
| plate 3, pos.21
| EcoRI/PstI + Sequenced Okay
|
| Const. Promoter + GVP (no.2)
| Low constitutive promoter with GVP cluster
| BBa_J23109 + BBa_I750016
| ~6100
| pSB1AC3
| Ampicillin/Chloramphenicol
| plate 3, pos.22
| EcoRI/PstI + Sequenced Okay
|
| Const. Promoter + GVP
| Medium constitutive promoter with GVP cluster
| BBa_J23106 + BBa_I750016
| ~6100
| pSB1AC3
| Ampicillin/Chloramphenicol
| plate 3, pos.23
| EcoRI/PstI + Sequenced Okay
|
| Const. Promoter + GVP (no.2)
| Medium constitutive promoter with GVP cluster
| BBa_J23106 + BBa_I750016
| ~6100
| pSB1AC3
| Ampicillin/Chloramphenicol
| plate 3, pos.24
| EcoRI/PstI + Sequenced Okay
|
| HmtA (1.1)
| HmtA-His in pBAD(a)
| None (to become BBa_K190018)
| 2004
| pBAD
| Ampicillin
| plate 3, pos.28
| Restriction(+)
|
| HmtA (2.1)
| HmtA-His in pBAD(a)
| None (to become BBa_K190018)
| 2004
| pBAD
| Ampicillin
| plate 3, pos.29
| Restriction(+)
|
| RBS MBPArsR
| BBa_B0034 + BBa_K190027
| None (to become BBa_K190018)
| ~1600
| pSB1A2
| Ampicillin
| plate 3, pos.30,31,32
| Restriction(+)PCR
|
| pLac-fMT + Ars-RFP
| pArs testing
| BBa_K190032 BBa_K190023 BBa_J61002 (without the backbone)
| ~1400
| pSB1A2
| Ampicillin
| plate 3, pos. 33,34,35
| Quality control
|
| CueO- + GVP in J61035
| CueO promoter without RBS in front of the GVP cluster
| None
| 6149
| BBa_J61035 (without the part between EcoRI and XbaI)
| Ampicillin
| plate 3, pos.37
| Restriction(+) + Sequenced Okay
|
| ZntR- + GVP in J61035
| ZntR promoter without RBS in front of the GVP cluster
| None
| ~6150
| BBa_J61035 (without the part between EcoRI and XbaI)
| Ampicillin
| plate 3, pos.38
| Restriction(+) + Sequenced (3 mutations)
|
| ArsR- + GVP in J61035
| ArsR promoter without RBS in front of the GVP cluster
| None
| ~6150
| BBa_J61035 (without the part between EcoRI and XbaI)
| Ampicillin
| plate 3, pos.39
| Restriction(+) + Sequenced Okay
|
| ArsR- in J61002
| pArsR (promoter without RBS)
| BBa_K190015
| 71
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.40
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
|
| ZntR- in J61002
| pZntR (promoter without RBS)
| BBa_K190016
| 65
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.41
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced (3 mutations)
|
| CueO- in J61002
| pCueO (promoter without RBS)
| BBa_K190017
| 43
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.42
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
|
| ZntR+RBS in J61002
| pZntR (promoter with own RBS)
| BBa_K190022
| 91
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.43
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
|
| ArsR+RBS in J61002
| pArsR (promoter with own RBS)
| BBa_K190023
| 81
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.44
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
|
| CueO+RBS in J61002
| pCueO (promoter with own RBS)
| BBa_K190024
| 71
| BBa_J61002 (without the part between EcoRI and SpeI)
| Ampicillin
| plate 3, pos.45
| EcoRI/SpeI(+), EcoRI/PstI(+), Sequenced Okay
|
| SmtA-GST in pGEX-3x
| SmtA-GST fusion
| no biobrick primers available
| 1400
| pGEX-3x
| Ampicillin
| plate 3, pos. 46
| PCR, restriction
|
| SmtA in pET29a
| SmtA
| BBa_K190021
| 900
| pET29a
| Kanamycin
| plate 3, pos. 47
| PCR, restriction
|
| MymT in pGB68
| MymT
| BBa_K190020
| 215
| pTXB1 (NEB)
| Ampicillin
| plate 3, pos. 48
| PCR, restriction
|
| GlpF and fMT in pMAL-MT
| GlpF and fMT on a synthetic operon in the vector
| BBa_K190019 and BBa_K190028
| fMT = 200, GlpF = 900?
| pMALc2x (NEB)
| Ampicillin
| plate 3, pos. 49
| PCR, restriction
|
| MBP-ArsR
| MBP-ArsR Fusion protein
| BBa_K190027
| 15180
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 53
| Restriction analysis & PCR
|
| pNL29
| GVP Gene cluster
|
| 6000
| pBluescriptIIKS
| Ampicillin
| plate 3, pos. 54
| Buoyancy phenotype
|
| pLac-fMT
| Metallothionein with inducible promotor
| BBa_K190032
| 430
| pSB1A2
| Ampicillin/chloramphenicol
| plate 3, pos. 55
| Quality control
|
| pLow-fMT #4
| Metallothionein with low constitutive promotor
| BBa_K190031
| 265
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 56
| Quality control
|
| pLow-fMT #3
| Metallothionein with low constitutive promotor
| BBa_K190031
| 265
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 57
| Quality control
|
| SmtA
| Metallothionein
| BBa_K190021
| 678
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 58
| Quality control
|
| fMT #6
| Metallothionein for As3+
| BBa_K190019
| 222
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 59
| Sequence 2 deletions between EcoRI and XbaI sites
|
| fMT #4
| Metallothionein for As3+
| BBa_K190019
| 222
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 60
| Sequence Okay!
|
| pZntR-GVP
| Metal promoter
| BBa_K190034
| Base Pairs (bp)
| pSB2K3
| Kanamycin
| plate 3, pos. 69
| Quality control
|
| pCueO-GVP
| Metal promoter
| BBa_K190035
| Base Pairs (bp)
| pSB2K3
| Kanamycin
| plate 3, pos. 70
| Quality control
|
| pLacI-GVP no.1
| Inducible promoter
| BBa_K190036
| Base Pairs (bp)
| pSB1A2
| Ampicillin
| plate 3, pos. 71
| Quality control
|
| pLacI-GVP no.2
| Inducible promoter
| BBa_K190036
| Base Pairs (bp)
| pSB1A2
| Ampicillin
| plate 3, pos. 72
| Quality control
|
| High consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23100
| Base Pairs (bp)
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 75
| Quality control
|
| Medium consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23106
| Base Pairs (bp)
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 76
| Quality control
|
| Low consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23109
| Base Pairs (bp)
| pSB1AC3
| Ampicillin/chloramphenicol
| plate 3, pos. 77
| Quality control
|
| High consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23100
| Base Pairs (bp)
| pSB3K3
| Kanamycin
| plate 3, pos. 78
| Quality control
|
| Medium consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23106
| Base Pairs (bp)
| pSB3K3
| Kanamycin
| plate 3, pos. 79
| Quality control
|
| Low consitutive promoter(with RFP)
| Constitutive promoter
| BBa_J23109
| Base Pairs (bp)
| pSB3K3
| Kanamycin
| plate 3, pos. 80
| Quality control
|
| Reference constitutive promoter)
| Constitutive promoter
| ?
| Base Pairs (bp)
| ?
| ?
| plate 3, pos. 81
| Quality control
|
| ArsR-GvP
| Ars promotor + GvP gas vesicle cluster
|
| ?
| ?
| Kanamycin
| plate 3, pos. 64
| ?
|
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