Team:KULeuven/21 September 2009

From 2009.igem.org

Project progress

Progress of parts

[edit] Blue Light Receptor

  1. a new restriction digest on logA (1&2) was done.
  2. the re-enting of likA was succesfull and the liquid culture was miniprepped. likA: 179,3 ng/µl. a restriction digest with EcoRI and PstI was performed and put on gel. the signals were good.
  3. a glycerol stock of ligY was made and some samples for sequencing were prepared.
  4. a motherplate of LogA en LikA were made and liquid cultures to make a glycerol stock.
  5. liquid cultures were also made for empty top10 cells, standard promotor and LikA (blp and GFP in pSB3K3).

[edit] Vanillin Production

  • Plating of Pams&co
  • Miniprepped electroporated PEFT
part concentration 260/280 260/230
PEFT1 1 UV 155,4 1,99
PEFT1 2 UV 158,8 2,00
PEFT2 1 UV 173,1 1,96
PEFT2 2 UV 149,5 2,05
  • All sizes where correct
  • Made motherplate from PEFT1 UV
  • Made motherplate from PAMS
  • Made motherplate from EFT

[edit] Vanillin Receptor

  • Again PstI din't cut overnight in both samples. A new restriction digest was done over one day because pstI did need maye more time to cut.
  • Electroporation of virB showed good results so motherplates were made

[edit] Key/Lock/Anti-Key