Team:KULeuven/24 August 2009

From 2009.igem.org

Project progress

Progress of parts

[edit] Blue Light Receptor

  1. Plates with LigA were put under blue light. The LEDs were put on their max capacity.
  2. Restriction digest with
    • tubes (1,3,5,7,9) of LigA (BLP + BBa_E0240) cut with EcoRI en PstI
    • promotor BBa_J23101 cut with EcoRI en SpeI (4x)
  3. Gel electrophoresis with the RD of LigA and BBa_J23101 followed by a gel extraction:
    • Note: tube 5 of LigA was loaded poorly on the gel and could not be used.
    • Note: the samples of promotor BBa_J23101 were barely visible. Only 2 of the 4 samples were recovered by extraction.
Part concentration (ng/μl) 260/280 λ
LigA 1 7,9 2,16
LigA 3 10,8 1,88
LigA 7 4,8 2,49
LigA 9 8,0 3,02
BBa_J23101 (A) 20,8 1,70
BBa_J23101 (B) 12,8 2,57

4. Enting of liquid cultures with kanamycin and pSB3K3

[edit] Vanillin Production

  • Electroporation competent cells with EF and SAMS
  • Cells were plated out and grown overnight

[edit] Vanillin Receptor

  • TOPO vector with insert W showed some colonies. PCR + electrophoresis were performed for control but a bad and unclear picture was made so the result was not that visible
  • B and G vector were digested to perform gelextraction
  • B and G were put on a gel ==> length of the fragments is OK
  • Enting of R202 in fluid medium en tomorrow second mutation (427)

[edit] Key/Lock/Anti-Key