Team:KULeuven/25 August 2009

From 2009.igem.org

Project progress

Progress of parts

[edit] Blue Light Receptor

  • Miniprepping the liquid cultures with pSB3K3
Part concentration (ng/μl) 260/280 λ
pSB3K3 (1) 27,6 1,91
pSB3K3 (2) 47,7 2,08
  • Restriction digest of pSB3K3 with EcoRI and PstI
  • Gel electrophoresis and extraction of the RD of pSB3K3

--> failed. There was no signal at all

Vector insert
pSB1A3 + BBa_E0240 BBa_J23101
30ng -> 1,4μl 40,8ng -> 2μl

[edit] Vanillin Production

  • We have colonies!!
  • Lots of EF, enough of SAMS
  • We made fluid cultures and let them grow overnight

[edit] Vanillin Receptor

  • PCR mutagenesis from R on 427, PCR purification and gelelectrophoresis: no signal on the picture probably because enting didn't show satisfying results
  • Ligation van X and Y in K14 at 16°C
  • New enting of R202
  • W has been ented on solid agar, so miniprep and nanodrop are scheduled for today
  • Mutagenesis of W + PCR purification

[edit] Key/Lock/Anti-Key