Team:KULeuven/3 August 2009


Project progress

Progress of parts

[edit] Blue Light Receptor

[edit] Vanillin Production

We received the COMT, sam5 and sam8 from Edinburgh today. Unfortunately, the tube containing the sam5 + RBS (with the PstI site) was opened during transport and all DNA in it was lost. The others were transferred into competent cells by electroporation.

The cells were put into liquid LB to recuperate from the procedure, transferred onto LB/amp plates and grown overnight at 37°C.

We also made liquid cultures of the RBS + fcs and RBS + ech cultures so we can miniprep them tomorrow.

[edit] Vanillin Receptor

  • Agrobacterium tumefaciens strain pTiA6NC/A1011 was plated out on LB-medium.
  • Primers for virA, virG, promotor region virB and RpoA have arrived

[edit] Key/Lock/Anti-Key