Team:KULeuven/Design/Vanillin Receptor

From 2009.igem.org

Vanillin Receptor: Design

Biologie vanillin receptor.png


The vanillin receptor senses the vanillin concentration outside the cell. This information is needed to create the feedback loop that controls vanillin synthesis.

Certain virulent bacteria like Agrobacterium Tumefaciens contain a large tumor-inducing plasmid that carries different virulence (vir) genes. The vanillin receptor is composed of two of those vir genes: virA Status: 500 Content-type: text/html

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and virG Status: 500

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Software error:

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. rpoA (Status: 500 Content-type: text/html

Software error:

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) is an alfa subunit polymerase that helps virG work in E. coli. These three genes are constitutively transcribed under promoter Status: 500 Content-type: text/html

Software error:

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. The terminator is Status: 500 Content-type: text/html

Software error:

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and Status: 500

Content-type: text/html

Software error:

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is chosen as RBS.

Together, virA and virG form a two component system that recognizes phenol derivatives like vanillin and other signals like low pH, certain aldose monosaccharides and limited phosphate. Under the influence of vanillin, virA is autophosphorylated. The virA dimers function as a sensor kinase. They process all the input signals and phosphorylate virG. VirG then binds to a vir box sequence located in the virB promoter region Status: 500 Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
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and triggers the transcription of antikey Status: 500

Content-type: text/html

Software error:

Can't locate Bio/SeqIO.pm in @INC (@INC contains: /synbio/lib /web-site/parts.igem.org/cgi/lib /usr/local/lib64/perl5 /usr/local/share/perl5 /usr/lib64/perl5/vendor_perl /usr/share/perl5/vendor_perl /usr/lib64/perl5 /usr/share/perl5 .) at /web-site/parts.igem.org/cgi/lib/Part.pm line 9.
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.