Team:KULeuven/Notebook/Key/Lock/AntiKey

From 2009.igem.org

Miss Blue key lock.png



Contents

Key / Lock / AntiKey Notebook

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

[edit] Thursday

Plating the glycerol stock strain containing Content-type: text/html

Software error:

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.

Week 3: 20 July 2009 - 26 July 2009

[edit] Wednesday

Liquid culture of Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

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has been made.

[edit] Thursday

The plasmid of Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

has been isolated from the liquid culture.

The glycerol stock strain containing Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

has been plated.

[edit] Friday

The glycerol stock strain containing Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

has been plated.

The key+terminator sequence, the lock sequence and the antikey+terminator sequence have all been ordered at GeneArt.

Week 4: 27 July 2009 - 2 August 2009

[edit] Monday

Following parts were put in liquid medium:

  • Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

+ Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

GFP with LVA tag and terminators
  • Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

TetR-promoter

[edit] Tuesday

Following plasmids were purified:

  • Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

GFP with LVA tag
    • concentration: 94,1 ng/μl
    • 260/280: 1,94
  • Content-type: text/html

Software error:

Can't call method "errstr" on an undefined value at /web-site/partsregistry.org/cgi/lib/BBDB.pm line 35.

For help, please send mail to this site's webmaster, giving this error message and the time and date of the error.

tetR-promoter
    • concentration: 98,2 ng/μl
    • 260/280: 1,89

Week 5: 3 August 2009 - 9 August 2009

Week 6: 10 August 2009 - 16 August 2009

Week 7: 17 August 2009 - 23 August 2009

Week 8: 24 August 2009 - 30 August 2009

Week 9: 31 August 2009 - 6 September 2009

Week 10: 7 September 2009 - 13 September 2009

Week 11: 14 September 2009 - 20 September 2009

Week 12: 21 September 2009 - 27 September 2009

Week 13: 28 September 2009 - 4 October 2009

[edit] Tuesday

  • We finally received the lock and the antisense key from geneart.
  • Electroporation and plating out the two components.

[edit] Wednesday

  • Liquid culture of the two components has been made

[edit] Thursday

  • Miniprepped and nanodropped
sample ng/µl
L1 93,5
L2 81,1
AK1 75,3
AK2 72,8
  • Restriction digest overnight: enzymes EcoRI and PstI

[edit] Friday

  • Gel electrophoresis and purification
  • Ligation of lock and antisense key in pSB1A2 (over weekend)

Week 14: 5 October 2009 - 11 October 2009

[edit] Monday

  • Electroporation of the ligation
  • Plating out the electroporated cells

[edit] Tuesday

  • No growth visible

[edit] Wednesday

  • Therefore new attempt to ligate the two components in iGEM vectors
  • Restriction digest: EcoRI and PstI

[edit] Thursday

  • Gel electrophoresis and purification
  • Ligation (overnight): both lock and antisense key are ligated in pSB1A2 and pSB3K3

[edit] Friday

  • Electroporated and plated out the ligations

[edit] Sunday

  • Liquid culture (the lock and antisense key in iGEM vectors)

Week 15: 12 October 2009 - 18 October 2009

[edit] Monday

Miniprep and nanodrop

  • Lock (pSB1A2): ng/µl
  • Antisense key (pSB3K3): ng/µl