Team:KULeuven/Used Parts

From 2009.igem.org

Individual parts

PartnumberShort description Resistance StatusLocationAdditional notes

J23066
Ribokey A/A Ordered at GeneArt
antisense
K238005
antikey A/A Ordered at GeneArt/ Received

J23078
lock Ap Ordered at GeneArt/ Received

B0015
Terminator Ak Available here -80 Can be found in -20 freezer

B0032
RBS Ap Available here -80

J23110
promotor Ap Available here -80 Can be found in -20 freezer

I742106
RBS + Sam8 A Received from Edinburgh -20
rbs + sam5
K238007
Sam5 A Received from Edinburgh -20
rbs+COMT
I742109
comt A/A Received from Edinburgh/available in iGEM plates -20

I742115
fcs Ap Received from Edinburgh -20

I742113
ech Ap Received from Edinburgh -20
VirA
K238008
VirA receptor Primers ordered This is purified from Agrobacterium tumefaciens
VirG
K238009
VirG activator Primers ordered This is purified from Agrobacterium tumefaciens
rpoA
K238010
RpoA Primers ordered This is purified from Agrobacterium tumefaciens and is necessary for VirG to work in E.coli
VirB
K238011
VirB promoter region Primers ordered This is purified from Agrobacterium tumefaciens and has virboxes that bind VirG
blr promot
K238000
YcgF/E promotor Region purified from E.coli strain MC4100 -20 Blue light promotor region from [1]
BLp
K238013
YcgF/E promotor In progress of being made Exact blue light promotor from [1]
pSB3K3
pSB3K3
Plasmid backbone From distribution plates Kanamycine resistant plasmid
GFP
K145015
GFP reporter From last years glycerol stock GFP+LVA+terminator

K145201
TetR generator From last years glycerol stock Promotor+RBS+tetR+terminator
GFP report
E0240
GFP reporter From last years glycerol stock RBS+GFP+terminator

Composite parts

PartnumberShort description Resistance StatusLocationAdditional notes

K238001
VirB promotor + key Biobrick in registry

K238002
Blr promotor + key Biobrick in registry

K238003
Vanillin receptor Biobrick in registry

K238004
Vanillin synthesis Biobrick in registry 5 paths in pathway never tested together in E. coli

K238006
Short Vanillin synthesis Biobrick in registry Short version containing the last 2 genes of the pathway, these have been tested in E. coli

K238012
Short Vanillin synthesis II Biobrick in registry Short version containing the first 3 parts of the pathway, these have not been tested in E. coli