Overview
- Integrated Heavy Metal Detection System
- Our team project is designing synthetic modules for simultaneous detection of multiple heavy metals such as
- arsenic, zinc, and cadmium in E. coli. The ultimate goal is to build a micromachine sensing and determining of
- the concentration of heavy metals in a sample solution (e.g. the waste water). In order to design the system,
- we will employ two fluorescence proteins (GFP and RFP) and aryl acylamidase as signal reporters. Since each
- heavy metal promoter produces unique fluorescence or color by those reporters, if more than two heavy metals
- coexist in a solution, the results would be interpreted from the convoluted fluorescence and/or color rather
- than a single signal detection. The successful construction of the synthetic modules in E. coli can be utilized
- in the form of a lyophilized powder, which can be stored in a drug capsule to make it portable.
- STEP1, Prepare plasmids.
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- STEP2. Gene cloning & Infusion Biobrick assembly(SLIC)
- Using the SLIC method, infuse the biobricks inside of plasmid. There are two inserts just made through SLIC method.
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- STEP3.measuring the concentration of heavy metal
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- This picture is showing cadimum detection. Another case is similar to this experiments.
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