Team:Kyoto/GSDD/Notebook/0914-0920

From 2009.igem.org

  1. Home
  2. GSDD
  3. Notebook

0727-0802
Construction.
0803-0809
Construction.
0810-0816
Construction.
0817-0823
Construction.PCR.
0824-0830
Construction.PCR.
0831-0906
Construction.PCR.MPR.
0907-0913
Construction.PCR.MPR.
0914-0920
Construction.PCR.MPR.
0921-0927
Construction.PCR.MPR.
0928-1004
Construction.PCR.MPR.
1005-1011
Construction.PCR.MPR.
1012-1018
Construction.MPR.Observation.

0914-0920 : Construction.PCR.MPR.


Monday, 14 August

To Do

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check

Results

p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
  • Gel extraction kit

Kyoto 0914 PCR.png

Miniprep product conc.
NO.Sample nameconc./(ng/ul)
1p-2-(1)9.3
2p-2-(7)25.9
3p-2-(8)8.6
4p-2-(10)7.5
4-(1)(2)(3)

Kyoto 0914 PCR 2.png

  • This result indicates that (1)a, (2)b and (3)a were inserted properly.



Tuesday, 15 September

To Do

Further to 14 Sept.

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check



Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)