Team:NYMU-Taipei/Project/Testing

From 2009.igem.org

Contents

7/14 taq virus protein


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
taq 0.25μl
ddH20 39.75 μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 150
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb+100bp
1: gp120 full length 2621bp
2: gp120 a-b 2539bp
3: H1N1-HA full length 1742bp
4: H5N1-HA full length 1742bp
5: H1N1-NA full length 1460bp
6: H5N1-NA full length 1391bp
7: H5N1-NA a-b 1283bp
8: Positive Control BBa_E0240 1114bp (876+238) 238bp
9: H5N1-HA b-c 911
10:H1N1-NA c-d 606bp
11:H1N1-NA d-e 495bp
12:Positive Control pLac 437bp
13:H5N1-HA d-e 378bp
14:H1N1-NA b-c 315bp
15:H5N1-HA a-b 285bp
16:marker 100bp



Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
taq 0.25μl
ddH20 39.75 μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 30
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0:marker 100bp
1:H5N1-HA c-d 236bp
2:gp120 b-c 114bp
3:H5N1-NA b-c 126bp
4:H1N1-NA a-b 103bp
5:negative control


7/20 pfu virus proteins


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-3: gp120 b-c 114bp
4-6: H5N1-HA c-d 236bp
7:marker 1kb



Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-2: H5N1_HA a-b 285bp
3-4: H5N1-HA d-e 378bp
5: marker 1kb
6: Positive Control BBa_E0240 1114bp (876+238) 238bp
7: negative control



Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 150
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-2: H5N1-HA b-c 911bp
3-4: gp120 a-b 2539bp
5: marker 1kb


7/20 taq virus proteins


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
taq 0.25μl
ddH20 39.75 μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1: marker 100bp
2: H1N1-HA full length 1742bp
3: H1N1-NA full length 1460bp
4: H5N1-NA full length 1391bp
5: H5N1-NA a-b 1283bp
6: H1N1-NA c-d 606bp
7: H1N1-NA d-e 495bp
8: H1N1-NA b-c 315bp
9: H1N1-NA d-e 495bp
10: H1N1-NA a-b 103bp
11: Positive Control BBa_E0240 1114bp (876+238) 238bp
12: negative control
13: marker 100bp
14: marker 1kb


7/21 taq H1N1-HA H5N1-NA gp120b-c


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
taq 0.25μl
ddH20 39.75 μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1:-
2: H5N1-NA a-b 1283bp
3: H5N1-NA full length 1391bp
4: H1N1-HA full length 1742bp
5: Positive Control BBa_E0240 1114bp (876+238) 238bp
6: -
7: marker 100bp


Descr Win length Fail length
0: marker 1kb
1: -
2: -
3: H5N1-NA b-c 126bp
4: gp120 b-c 114bp
5: negative control
6: -
7: marker 100bp

7/22 pfu virus protein


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-2: H1N1_NA a-b 103bp
3-4: gp120 b-c 114bp
5-6: H1N1-NA b-c 315bp
5-6: marker 100bp



Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-2:H1N1-NA d-e 495bp
3-4:H5N1-HA c-e 591bp
5-6:H1N1-NA c-d 606bp
7: marker 100bp


Descr Win length Fail length
0: marker 1kb
1-2:H5N1-NA a-c 1175bp
3: Positive Control BBa_E0240 1114bp (876+238) 238bp
4: negative control
5: marker 100bp


7/23 pfu virus protein


Mix: 50μl
template 1μl
forward primer - 1μl
reverse primer - 1μl
dNTP 2μl
10x buffer 5μl
pfu 0.5μl
ddH20 39.5μl
Protocol:
1. 94oC: 60
2.
30 cycles 		94oC: 15
55oC: 20
72oC: 120
3. 72oC: 300
2% agarose, 90V, 30min

Descr Win length Fail length
0: marker 1kb
1-2:H1N1-HA 1742bp
3-4:H5N1-HA 1742bp
5-6:H5N1-NA a-b 1283bp
7: Positive Control BBa_E0240 1114bp (876+238) 238bp


Descr Win length Fail length
0: marker 1kb
1-2:H1N1-NA c-e 1081bp
3-4:H1N1_NA a-c 398bp
5-6:H5N1_NA b-c 126bp
7: negative control
Retrieved from "http://2009.igem.org/Team:NYMU-Taipei/Project/Testing"