"
Team:Newcastle/IntroductoryLabwork/1 July 2009
From 2009.igem.org
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
| Previous | Next |
Introductory Lab Session: 1st July 2009
Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.
Things needed for today's session
Things we need for today include:
- LB
- Containers
- Sterile tubes
- Space in orbital shaking incubator for tonight
Progressing with making competent cells
- We plated out single cultures from yesterday's plates for a practice.
- We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
- We made up some 50% glycerol and it was sent to be autoclaved.
Preparations for next lab session
Considerations
- How much equipment (e.g. tubes) and solutions have we used today? Is there enough for Thursday's lab session?
Things we need for tomorrow (Thursday):
General
- Sterile tubes
- Pipette tips
- Solutions
'Culture' Step (from making competent cells protocol)
- Sterile flasks for culture (500ml)
- Spectrophotometer and cuvettes (where)
- Arrange space in shaking incubator
'Harvest cells' Step (from making competent cells protocol)
- Centrifuge/ centrifuge vial (200ml?)
- Ice bucket and ice
- Glycerol
- Sterile microfuge tubes
- Liquid nitrogen
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
