"
Team:Newcastle/IntroductoryLabwork/27 July 2009
From 2009.igem.org
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Introductory Lab Session - 27th July 2009
Introduction
Last week the team had completed the ethanol precipitation of the GFP and RFP (in plasmids pSB1A2 and pSB1AT3 respectively) DNA samples. They are midi-prepped plasmid DNA samples extracted using Sigma-Aldrich's GenElute Midi-prep kit, however the ethanol present in Wash Solution 2 hadn't been completely removed so ethanol precipitation was necessary. With the ethanol removed, it should be analyse the samples through DNA gel electrophoresis.
Practical Outline
- Run the GFP and RFP plasmid DNA samples on gel through DNA gel electrophoresis and analyse.
Analysing the DNA samples through DNA Gel Electrophoresis
|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
