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Team:Newcastle/Labwork/14 October 2009
From 2009.igem.org
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Formal Lab Session - 14th October 2009
- This morning we check yesterday's transformation result, it turned out we got transformed cell for all three strains.
- we patched the colonies on LB+EM+CHL+Starch plate to check whether the pGFP-rrnB:kinA part were integrated in amyE part or not.
- After 6 hours grow on antibiotic and starch plate, we got some right colonies.
Microscopy Test to characterize KinA
We inoculated 6 cultures for the microscopy test from haloless colonies since tehy were transformed with our biobrick. We inoculated them into MM competence medium overnight
- Colony 1 from BFS2426 transformation
- Colony 18 from BFS2426 transformation
- Colony 13 from BFS40 transformation
- Colony 18 from BFS240 transformation
- Colony 19 from BFS867 transformation
- Colony 22 from BFS867 transformation
- Colony 5 from BFS2426 with halo. (As a negative control)
- No cell (Control)
Transformations
We did transformations from midipreps to have stock for our biobricks. We will freeze them later. We transformed ara, sac, ara+sspB Biobricks.
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News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
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