Team:PKU Beijing/Notebook/AND Gate 2/T3

From 2009.igem.org

 
Notebook > AND_Gate_2 > T3

2009.7.30

12:30 T7 Polymerase store the strain
13:00 T3 Promoter + GFP Double digestion check
13:00 T3 Promoter Parts Double digestion check
13:45 T7 Polymerase Miniprep

2009.8.1

15:00 T7 Polymerase + 1-2M check

2009.8.5

18:20 T3 Promoter PCR

2009.8.6

21:30 Gel recycle of T3 Promoter PCR
23:45 T3 Promoter PCR Product Double digestion

2009.8.7

11:00 Purify of T3 Promoter Digested product
11:00 Three Counter Plasmid Miniprep
15:30 T3 Polymerase High pure PCR
18:00 Gel recycle of T3 Polymerase PCR

2009.8.8

11:00 Bi-stable Miniprep
12:00 Bi-stable Plasmid Double Digestion

2009.8.9

21:00 Ligation of T3 Promoter (insert) and PSB1K3 (Vector)
21:15 T3 Polymerase PCR Product Double Digestion

2009.8.10

6:00 T3 Polymerase Digestion Product Purify
6:30 Ligation of T3 Polymerase (insert) and PSB1K3 (Vector)
10:00 Transformation of T3 Promoter
22:00 Transformation of T3 Polymerase

2009.8.11

4:00 Sake the T3 Promoter Parts
4:00 PCR of T3 Promoter Parts
16:30 T3 Promoter Parts Miniprep

2009.8.13

13:30 Miniprep of T3 Polymerase
14:30 Double digestion of T3 Polymerase plasmid

2009.8.15

12:00 Minioreo of T3 Polymerase
14:15 Double digestion of T3 Polymerase plasmid
15:00 Sake the T3 Promoter Parts
20:00 Sake the T3 Polymerase

2009.8.16

14:15 Miniprep of T3 Promoter Parts
16:00 Miniprep of T3 Polymerase

2009.8.17

10:30 Double digestion of T3 Promoter Parts and T3 Polymerase
11:00 Miniprep of 3 Counter Plasmid and T3 Polymerase
22:00 Sake the T3 Polymerase

2009.8.18

12:00 Miniprep of T3 Polymerase
15:00 Double digestion of T3 Polymerase plasmid

2009.8.20

10:00 PCR of T3 Polymerase
14:00 PCR of T3 Promoter + GFP
17:00 PCR of T3 Promoter
20:00 Cut Gel

2009.8.22

12:00 Confirm the PCR product of T3 Promoter

2009.8.23

13:00 Gel cycle of T3 Polymerase and T3 Promoter + GFP
15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP
21:00 Ligation of digested T3 Polymerase and T3 Promoter + GFP
23:00 Transformation of T3 Polymerase and T3 Promoter + GFP

2009.8.25

22:00 Shake the T3 Polymerase and T3 Promoter + GFP

2009.8.26

11:00 Miniprep of the T3 Polymerase and T3 Promoter + GFP
15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP

2009.8.27

0:00 Shake the T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)
13:00 Miniprep of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)

2009.8.28

10:00 Single Point Mutation of T3 Polymerase
20:00 Ligation of Mutated T3 Polymerase
21:00 Transformation of Mutated T3 Polymerase

2009.8.29

12:00 Double digestion of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)
16:00 Transformation of CI Promoter Parts

2009.8.30

16:00 Double digestion of T3 Polymerase and T3 Promoter + GFP
20:00 Shanke CI Promoter and 3 Counter
21:00 Cut Gel of T3 Polymerase

2009.9.2

11:30 Gel cycle of T3 Polymerase
14:00 Miniprep of 3 Coumter Plasmid

2009.9.4

12:00 Ligation of T3 Polymerase and 1-2M
16:00 Transformation of T3 Polymerase and 1-2M
20:00 PCR of T3 Polymerase (long primer)

2009.9.6

11:00 Shake the T3 Polymerase + 1-2M
15:00 Cut Gel of PCR Product of T3 Polymerase
15:30 Gel cycle

2009.9.7

11:00 Double digestion of T3 Polymerase PCR Product
11:30 Double digestion of T3 Promoter + GFP

2009.9.11

11:00 Shake 1-1M
12:00 Double digestion of T3 Promoter + GFP (High concerntration)

2009.9.13

12:00 Miniprep of 1-1M
16:00 Gel cycle of Double digestion of T3 Promoter + GFP (High concerntration)

2009.9.14

12:00 Single digestion to ensure the T3 Promoter + GFP Plasmid
16:00 Miniprep of 1-1M

2009.9.16

13:00 Double digestion of 1-1M

2009.9.18

13:00 Double digestion of 1-1M (High Concerntration)

2009.9.20

9:00 Cut Gel of 1-1M
9:15 Gel cycle
11:00 Shake T3 Promoter + GFP

2009.9.21

13:00 Miniprep of T3 Promoter + GFP
16:00 Double digestion of T3 Promoter + GFP

2009.9.22

10:00 Ligation of T3 Polymerase and 1-1M
18:00 Transformation of T3 Polymerase

2009.9.23

20:30 Shake T3 Polymerase +1-1M



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