Team:PKU Beijing/Notebook/Protocol/PCR


Notebook > Protocol > PCR with EasyPfu DNA Polymerase

Protocol for PCR with EasyPfu DNA Polymerase

download PDF version

Preparation for Reaction Mixture

ReagentFinal concentrationVolume
10X Taq buffer1X2 µl
2.5 mM dNTP mix0.25 mM2 µl
Forward Primer0.2-0.4 µM1 µl
Reverse Primer0.2-0.4 µM1 µl
EasyPfu DNA Polymerase25 units1 µl
Template DNAAs required<0.5 µg
ddH2Oto final volume

Cycling Conditions

Initial Denaturation945
Primer AnnealingAs requiredAs required
Extending72As required(0.5kb/min)
Final Extending7210
Number of Cycles 32-35
Cooling Down410