Team:Paris/27 July 2009
From 2009.igem.org
NoteBook
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Lab work
- Media: 15 LB agarosis ampiciline dishes were made.
- PCR for oligo P1-P2 (tolR); P3-P4 (tolA); P5-P6 (tatABC); P7-P8 (clyA fusion).
- Matrice is Genomic DNA of [S2] K12 1655 ΔrecA::Kan. PCR Quick load protocol
- PCR launched with a Tm at 60°C (programme quick load).
- Buffer for Competent bacteria by RbCl
- Buffer I (250ml)
- Buffer II (125ml)
- DH5 alpha competent bacteria by RbCl