Team:TUDelft/7 August 2009

From 2009.igem.org

Lab Notebook

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August
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7 August 2009

Sriram

I got colonies in all plates of heat shock transformation and electro-transformation. All must be checked for their sizes on monday. If it is ok then I can go to the next step of assembly.

Tim Weenink

Did the rest of the assembly transformations. This time TSS and RubidiumCloride chemically competent cells were transformed with all ten constructs. LB medium was used instead of SOC, because all the SOC was used yesterday.


Calin

The long knockout primers and trbK gene arrived. Designed short knockout primers. CA had colonies. But not all expressed GFP:

Set GFP colonies Non-GFP colonies
RbCl 3 140
TSS 1? 7
ElectroPoration 6 54

Made a backup AMP plate for trbK. Rehydrated and electroporated BBa_J13002 (pTet-RBS). Should be able to save some assembly steps by using this part

Set Time Constant
Assembly 1 4.4
BBa_J13002 4.6

Daniel made CAM control plates at both high and low concentrations.