Team:TorontoMaRSDiscovery/14 August 2009

From 2009.igem.org


August 14, 2009

  1. Miniprepped 5, 7, C with normal protocol of BioBasics Kit
    • Results were low ~30ng/ul
  2. Digested C and CIPed it
  3. Ran 4 digest and plasmid samples of 5, 7, C and the CIPed C plasmid samples of 5, 7, C and the CIPed C plasmid
  4. Gel extracted CIPed C plasmid
  5. Ligated 4+5 and Enc into C plasmid and plated