Team:TorontoMaRSDiscovery/1 September 2009

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September 1, 2009

  1. only 3+2a overnight showed growth
  2. Miniprepped 3+2a
  3. digested 3+2a, 4+5R, 3, 4, 5 with E,P
  4. Transfected EncC+5 and negative control into DH-5 cells, plated on Tet plates
  5. mistakenly tranfected T, K plasmid into DH-5 cells (ccdb gene)
  6. Ran digests on a gel
    • 3+2 still not confirmed, but all 4+5 samples showed expected band
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