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From 2009.igem.org

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September 24, 2009

1. Made a new batch of chemically competent cells
   • poured cells into 50ml falcon tubes
   • 350ul aliquots + 350ul 40% glycerol
2. Started 12E overnights from transfections done yesterday
   • Named: 1-5, a-e, but these have been used already, hence 1-5 were renamed 6-10, a-e renamed f-j
3. Transformed ligation from Monday and negative control into new cells and plated on new K plates

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