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From 2009.igem.org

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August 26, 2009

1. 4+5 plates showed 2 big colonies and 1 small colony
2. Started log phase for 4+5 (1), (2) (did not stock)
3. 3+2 overnights did not grow
   • They are not C-resistant?
   • We have to redo the 3+2 ligations
4. Miniprepped Enc A, B, C, BB3 and BB4
5. Digested miniprepped samples and also BB2, 1+2 plasmid for 2 hours
6. Ran digests on a gel
   • 1ug of plasmid was digested for Enc A, B, C, BB2, BB3, BB4; and 1/5 was loaded on to gel (10ul of digest)
7. 30x37.4=1122ng of plasmid was digested for 1+2; and 16.5ul was loaded (1122x16.5/50=370.26ng)
8. Miniprepped log phase of 4+5 (1), (2)

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