Team:TorontoMaRSDiscovery/4 September 2009

From 2009.igem.org


September 4, 2009

  1. Miniprepped overnight cultures
  2. stocked Amp, K1, K2, and Enc+5 (2), (3), (4)
  3. Digested Enc+5 (1)-(4), K1,2, BB7
  4. Transfected 1+2+3+2 into DH-5 cells (50ul)
    • left on ice for 2.5h after adding ligation product
    • incubated on shaker for about 2h
  5. Started overnight of C plasmid (ccdb)