Team:TorontoMaRSDiscovery/8 August 2009


August 8, 2009

  1. BB1 transformants showed many colonies
    • plates (a plate full of colonies)
    • -> increasing heat shock to 90s increased efficiency (Note: 3x DNA used in this transformation)
    • Calvin mentioned he does heat shock for 120s
    • Calvin also suggested to increase tranformation time, ie. after adding DNA, hold on ice for an hour
  2. DH-5alpha replates all grew on the plates, demonstrating LB can be re-autoclaved and poured as plates
    • there was no difference between transformants directly plated after adding LB+glucose or incubating for an hour
  3. Miniprepped overnights (1+2) samle 1,2,4, and K