Team:UNIPV-Pavia/Notebook/Week4Sep

From 2009.igem.org

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December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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July 2009
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August 2009
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September 2009
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October 2009
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Week from September 21st, to September 27th, 2009

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September, 21st

  • Wiki updating

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September, 22nd

pH sensor
  • LB NaCl 70 mM pH 5,5 - 6,6 - 7,5 - 8,5 + Amp preparation.
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of these three cultures at 37°C, 220 rpm.


Experiment with Tecan F200

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September, 23rd

pH sensor

2nd experiment

  • We put 50 ul into 5 ml LB NaCl 70 mM + Amp pH 6,6 falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half at 37°C, 220 rpm.
  • OD measure of the three falcons with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
    • pNhaA into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
    • A2 into:
      • LB NaCl 70 mM + Amp pH 5,5
      • LB NaCl 70 mM + Amp pH 6,6
      • LB NaCl 70 mM + Amp pH 7,5
      • LB NaCl 70 mM + Amp pH 8,5
  • We started this 6 hours' experiment that performed absorbance and fluorescence measures (on three different wells) for each sample every 5 minutes.


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September, 24th

  • Fermentation experiment setup
  • Wiki updating

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September, 25th

pH sensor
  • Preparation of LB NaCl 151 mM at pH 5,5 - 6,6 - 7,5 - 8,5.
  • Preparation of LB NaCl 250 mM at pH 5,5 - 6,6 - 7,5 - 8,5.


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September, 26th

  • Fermentation experiment
  • Cloning
  • Wiki updating

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September, 27th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA
  • Overnight incubation of these three cultures at 37°C, 220 rpm.

Top



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