Team:UQ-Australia/Notebook/Colony pick procedure

From 2009.igem.org

Colony Picking Procedure

Micro-culture

3ml of LB Broth 3ml of Antibiotic (depends on the antibiotic resistance gene on the plasmid)

1. Touch a single colony with a pipette tip and place the entire tip into the tube

2. Repeat 5 times or for as many colonies which are present

3. Place in incubator/shaker overnight at 37'C