From 2009.igem.org
Promoter Characterization Project Planning
- Table of triplets to be composed together
| Promoter | Gene (includes RBS and Terminator) | Backbone | E. Coli type
|
| J23100
| E0240 | pSB3K3 | NEB10β
|
| J23101
| E0240 | pSB3K3 | NEB10β
|
| J23102
| E0240 | pSB3K3 | NEB10β
|
| J23103
| E0240 | pSB3K3 | NEB10β
|
| J23104
| E0240 | pSB3K3 | NEB10β
|
| J23105
| E0240 | pSB3K3 | NEB10β
|
| J23107
| E0240 | pSB3K3 | NEB10β
|
| J23110
| E0240 | pSB3K3 | NEB10β
|
| J23113
| E0240 | pSB3K3 | NEB10β
|
| J23116
| E0240 | pSB3K3 | NEB10β
|
| R0011
| E0240 | pSB3K3 | NEB10β
|
| R0040
| E0240 | pSB3K3 | NEB10β
|
- Table of BioBrick parts, concentrations and amounts
| Part No. | Concentration (microgram/milliliter) | Amount (microgram)
|
| J23100
| 14.0 | 5.32
|
| J23103
| 7.0 | 2.52
|
| J23105
| 8.0 | 2.96
|
| J23104
| 11.0 | 4.18
|
| J23101
| 12.0 | 4.56
|
| J23107
| 18.0 | 8.1
|
| J23116
| 20.0 | 7.2
|
| J23113
| 14.0 | 5.18
|
| J23110
| 12.0 | 4.2
|
| J23102
| 31.0 | 10.23
|
| R0011
| 21.0 | 5.46
|
| R0040
| 15.0 | 5.4
|
| pSB3K3 (Total)
| n/a | 5.465
|
| pSB3K3 (1)
| 24 | .984
|
| pSB3K3 (2)
| 30 | 1.236
|
| pSB3K3 (3)
| 31.5 | 1.310
|
| pSB3K3 (4)
| 45 | 1.935
|
| E0240 (Total)
| n/a | 6.574
|
| E0240 (1)
| 30.0 | 1.200
|
| E0240 (2)
| 37.5 | 1.575
|
| E0240 (3)
| 45.0 | 1.868
|
| E0240 (4)
| 49.5 | 1.931
|
- Let me know if this needs to go anywhere else. I will put them in order later. =)
MandM 23:39, 15 June 2009 (UTC)
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